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Identification and characterization of a zebrafish gluathione s-transferase pi
Contents
Abstract
Acknowledgments
Contents
List of Figures
1. Introduction 1
1.1 Overview of Drug Metabolism
1.2 Phase II Conjugating Enzymes
1.3 Glutathione conjugation and Glutathione S-transferases
1.4 Physiological Roles of the Glutathione S-transferases
1.4.1 The role of Glutathione S-transferases in Detoxification
1.4.2 Other roles of Glutathione S-transferases
1.5 Zebrafish as an animal model
1.6 Objectives and goals
2. Materials and Methods
2.1 Materials
2.2 Methods
2.2.1 Bioinformatics
2.2.2 Cloning, Bacterial Expression, and Purification of Recombinant Zebrafish GST Pi
2.2.3 Analysis of the development stage-dependent expression of the zebrafish GST Pi
2.2.4 Miscellaneous methods
2.2.5 GST Pi Enzymatic Assay
2.2.6 pH-Dependency studies
3. Results
3.1 Identification of zebra fish GS sequence
3.2 Molecular Cloning, Expression, and Purification of the Zebrafish GST Pi
3.3 Analysis of the development stage-dependent expression of the zebrafish GST Pi
3.4 The pH-Dependency studies
4. Discussion
ReferencesBài viết liên quan
