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Investigating mechanical strain-induced phenotypic changes on prostate cancer cell toward metastasis using a three-dimensional in-vitro model
Table of Contents
Abstract
Acknowledgements
Table of Contents
List of Figures
List of Abbreviations
1 Introduction
2 Mechanical Strain and Mechanical Loading
2.1 Introduction
2.2 Mechanical Stress
2.3 Mechanical Strain
2.3.1 Uniaxial Stretch
2.3.2 Out-of-Plane Biaxial Stretch
2.3.3 In-Plane Biaxial Stretch
2.4 Conclusion
3 A Novel Uniaxial Mechanical Loading Platform: UNIcycler
3.1 Introduction
3.2 Relevant Literature in Cellular Based 3D Uniaxial Stretch Devices
3.3 A Novel Mechanical Loading Platform: UNIcycler
3.3.1 Components of UNIcycler Mechanical Loading Platform
3.3.1.1 The Plate System
3.3.1.2 Eccentric Cam Mechanism
3.3.1.3 The Motor
3.3.2 Mechanical Strain Application
3.3.3 Cell Encapsulation Material Selection for UNIcycler
3.4 Contribution to UNIcycler System
3.5 Conclusion
4 Effect of Applied Uniaxial Strain on Prostate Cancer (PC3) Cell Metastatic Potential: Cell and Intracellular Level
4.1 Introduction
4.2 Relevant Literature in Mechanical Loading Systems for Cancer Research
4.3 Materials and Methods
4.3.1 PC3 Cell Encapsulated 3D Collagen Construct Preparation
4.3.2 Mechanical Loading of Cell-Collagen Construct
4.3.3 Effect of Mechanical Loading on Actin Cytoskeletal Organization
4.3.4 Effect of Mechanical Loading on Cell Elongation
4.3.4.1 Cell Elongation and Elongation Index Calculation
4.3.5 Effect of Mechanical Loading on Actin Polymerization
4.3.6 Effect of Mechanical Loading on PC3 Cell Migration Potential
4.3.7 Statistical Methods
4.4 Results
4.4.1 Effect of Mechanical Loading on Cytoskeletal Reorganization of PC3 Cells Embedded in Collagen Constructs
4.4.2 Effect of Mechanical Loading on Cell Morphology of PC3 Cells Embedded in Collagen Constructs
4.4.3 Effect of Mechanical Loading on Actin Polymerization of PC3 Cells Embedded in Collagen Constructs
4.4.4 Effect of Mechanical Loading on Migration of PC3 Cells Embedded in Collagen Constructs
4.5 Discussion
5 Conclusions and Future Work
5.1 Conclusions
5.2 Future Work
5.2.1 Role of Cyclic Uniaxial Stretch in Cell Metastatic Progression
5.2.2 Effect of Uniaxial Stretch on Mode of Cell Migration
5.2.3 Cell Migration to Secondary Environmental Sites
5.2.4 Effect of Increased Strain Magnitude, Application Frequency, and Strain Duration on Cell Metastatic Potential
References Bài viết liên quan
