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Naturally-occurring fusion between the regulatory and catalytic components of type IIP restriction-modification systems


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Naturally-occurring fusion between the regulatory and catalytic components of type IIP restriction-modification systems
Table of Contents
Abstract
Acknowledgements
Table of Contents
List of Figures
Chapter 1: Literature Review
1. Restriction-modification (R-M) systems
2. Roles and Control of R-M systems
3. PvuII R-M system and its regulatory characteristics
4. CR fusion protein in Type II R-M systems
Chapter 2: Materials and Methods
Chapter 3: Results
1. Identification of C-REase-fused R-M systems
2. Testing for translational frameshifting (free NsoJS138I C protein production)
3. Activity assay for assessing NsoJS138IR activity and specificity
4. In vivo test of CR.NsoJ138I for C protein activity
Chapter 4: Discussion and Conclusion
References 
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